The third-ranked Eagles topped DMACC by 20 shots, 603 to 623, in the 36-hole tournament at the Veenker Memorial Golf Course in Ames.
Kirkwood advanced to the NJCAA Division II national tournament May 21-24 at Swan Lake Resort in Plymouth, Ind.
James Morgan of Kirkwood claimed medalist honors at 3-over-par 147 with rounds of 71 and 76. Ben Epperly of Kirkwood placed third, Will Babcock tied for fifth, Ryan Morrison tied for seventh and Dawson Peters tied for 15th.
Kirkwood has won the regional title 14 times in the last 16 years. The Eagles also have produced the medalist 14 times in the last 16 years.
DMACC and Southwestern also qualified for the national tournament.
1. Kirkwood 603, 2. DMACC 623, 3. Southwestern 625, 4. Northeast 641, 5. NIACC 646, 6. Iowa Lakes 649, 7. Hawkeye 654, 8. Southeastern 673, 9. Iowa Central 716.